denaturing gel electrophoresis

Study protein-protein interaction by native and denaturing gel electrophoresis , western blotting , and immunology precipitation .

研究蛋白质-蛋白质相互作用，由本地和变性凝胶电泳免疫印迹，免疫沉淀。

Total RNA was extracted , then its purity was detected by ultraviolet spectrophotometer , and its integrity was tested by taking the total RNA samples to RNA formaldehyde denaturing gel electrophoresis . 4 .

提取总RNA，用紫外分光光度计法检测RNA的纯度，并取总RNA样品进行甲醛变性凝胶电泳，检测总RNA完整性。

Formaldehyde denaturing gel electrophoresis showed that all samples have clear 28S and 18S bands and a weak 5S strip . And the band of 28S is 1.5 times the intensity of 18S by visual observation . 3 .

甲醛变性凝胶电泳显示，各样品电泳图谱有清晰的28S、18S条带和一条浅的5S条带，且肉眼观察28S条带亮度约为18S的1.5倍。

According to RNA at A260 / 280 value ≥ 1.7 , as well as formaldehyde denaturing gel electrophoresis , 28s , 18s rRNA bands clarity , brightness ratio near 2:1 , to meet the requirements of miRNA chip experiments . 3 .

根据RNA在A260/280值≥1.7，以及甲醛变性凝胶电泳28s、18SRRNA条带清晰，亮度比值接近2：1（见图9），满足miRNA芯片实验的要求。

Denaturing gradient gel electrophoresis system used in mutations detecting

变性梯度凝胶电泳装置及其在DNA突变检测中的初步应用

Review on Denaturing Gradient Gel Electrophoresis and Its Application in Food Microbiology

变性梯度凝胶电泳技术及其在食品微生物研究中的应用

Analysis of mutant frequency of PCR cloning by denaturing gradient gel electrophoresis

用变性梯度凝胶电泳分析PCR克隆的突变率

Four novel point mutations of factor ⅸ gene detected by denaturing gradient gel electrophoresis

变性梯度凝胶电泳检测到四例新的FⅨ基因突变

Analysis of Fungal Communities Present in Morchella Growth Soil Using Denaturing Gradient Gel Electrophoresis

采用变性梯度凝胶电泳研究羊肚菌土壤真菌群落结构

Evaluation of oral microbial diversity with denaturing gradient gel electrophoresis

变性梯度凝胶电泳分析口腔微生物多样性的研究进展

A denaturing gradient gel electrophoresis ( DGGE ) system was designed .

设计了一套适用于变性梯度凝胶电泳（DGGE）的装置。

Detection of denaturing gradient gel electrophoresis in pathogenic bacteria of periodontitis

牙周炎致病菌的变性梯度凝胶电泳检测

Analysis of Microflora Diversity by Denaturing Gradient gel Electrophoresis

利用变性梯度凝胶电泳分析微生物的多样性

Denaturing gradient gel electrophoresis ( DGGE ) has been widely applied for study microbial population structures recent years .

DGGE（变性梯度凝胶电泳）技术目前被广泛应用于分析微生物的种群组成。

Using Denaturing Gel Gradient Electrophoresis to construct the standard finger printing of lactobacilli from healthy human feces

应用DGGE方法构建健康人粪便乳杆菌标准指纹图谱

Methods APC mutation was measured with multiplex PCR , denaturing gradient gel electrophoresis and DNA sequencing ;

方法采用多重PCR、DGGE电泳和DNA测序技术检测76例大肠癌APC基因15外显子突变；

The length polymorphism of AR gene was demonstrated by denaturing polyacrylamide gel electrophoresis and silver staining .

AR基因产物经变性聚丙烯酰胺凝胶电泳、银染显示其长度多态性；

Bacterial Diversity in Goat Rumen as Characterized by Denaturing Gradient Gel electrophoresis and 16S rDNA Sequence Analysis

应用变性梯度凝胶电泳技术和16Srdna序列分析技术研究山羊瘤胃细菌的多样性

The technique of denaturing gradient gel electrophoresis ( DGGE ) was used to analyze the changes of total microflora structure .

主要试验结果如下：1.利用变性梯度凝胶电泳（DGGE）技术分析肠道总菌群结构的变化。

Study the detection of p53 gene mutation on colorectal cancer and liver metastasis by denaturing gradient gel electrophoresis and automated sequencing

变性凝胶电泳和自动DNA序列分析检测结直肠肝转移癌p53基因突变的研究

PTEN LOH : PCR - SSCP denaturing PAGE gel electrophoresis and sliver staining .

PCR一SSCP变性聚丙烯酞胺凝胶电泳检测PTEN杂合性缺失。

Analysis of Mutations in Exon 3 of Phenylalanine Hydroxylase Gene from Southern Chinese PKU by Denaturing Gradient Gel Electrophoresis

变性梯度凝胶电泳分析PAH基因外显子3突变

The size of the duck ovalbumin mRNA characterized by denaturing agarose gel electrophoresis was 21s .

用变性的琼脂糖凝胶电泳对各纯化步骤的核酸样品进行组成分析，确定出鸭卵清蛋白mRNA的大小约为21S。

In this paper , several molecular methods such as fluorescence in situ hybridization ( FISH ) and denaturing gradual gel electrophoresis ( DGGE ) etc.

为此，在介绍变性梯度凝胶电泳（DGGE）以及荧光原位杂交（FISH）等分子生物学新技术的基础上，对这些技术在土壤微生态研究中存在的问题和发展前景做了展望；

Objective To investigate the changes of subgingival bacterial communities in periodontal pockets with different depth using denaturing gradient gel electrophoresis ( DGGE ) analysis .

目的应用变性梯度凝胶电泳（DGGE）方法分析经牙周基础治疗使牙周袋变浅后龈下微生物种类的变化。

Methods : PCR , denaturing gradient gel electrophoresis ( DGGE ) and DNA sequencing were used to screen mutations in the factor ⅷ gene .

方法：用PCR、变性梯度凝胶电泳（DGGE）和DNA测序检测因子Ⅷ基因突变。

Denaturing gradient gel electrophoresis ( DGGE ) of 16S rRNA genes revealed a dynamic change in the microbial community structure of the soil during bioremediation .

16SRRNA的变性梯度电泳（DGGE）结果揭示了生物修复过程中土壤微生物菌群结构中的动态变化。

Denaturing gradient gel electrophoresis ( DGGE ) is an important molecular polymorphism technique . It has been widely used in the study of species identification and plankton community composition .

变性梯度凝胶电泳技术（denaturinggradientgelelectrophoresis，DGGE）是一种重要的分子多态性技术，在物种鉴定及浮游生物群落多样性研究方面有着广泛的应用。

Four microsatellite markers linked to FecB locus on 6 chromosome in ovine were analyzed for 108 animals by PCR and denaturing polyacrylamide gel electrophoresis .

选择4个位于绵羊6号染色体上且与FecB基因紧密连锁的微卫星标记，对小尾寒羊基因组进行PCR扩增后，采用最小二乘法估计各等位基因片段对产羔数的影响。

Studies on the distribution of microbe and their degradation of organic material in compost are presented , and the character of denaturing gradient gel electrophoresis ( DGGE ) method is also discussed .

对当前堆肥中微生物种群分布及其对有机物分解作用的研究进行分析，论述了分子生物技术中的变性梯度凝胶电泳（DGGE）的特点。